Species - level resolution of 16 S rRNA gene amplicons sequenced 1 through the MinIONTM portable nanopore sequencer

Species - level resolution of 16 S rRNA gene amplicons sequenced 1 through the MinIONTM portable nanopore sequencer 2

Species-level resolution of 16S rRNA gene amplicons sequenced through the MinION™ portable nanopore sequencer

BACKGROUND The miniaturised and portable DNA sequencer MinION™ has been released to the scientific community within the framework of an early access programme to evaluate its application for a wide variety of genetic approaches. This technology has demonstrated great potential, especially in genome-wide analyses. In this study, we tested the ability of the MinION™ system to perform amplicon seq...

Species level resolution of 16S rRNA gene amplicons sequenced 5 through MinION portable nanopore sequencer

25 The miniaturized and portable DNA sequencer MinION has been released to the scientific community in the framework of an early access programme to evaluate its scope in a wide variety of genetic approaches. Although this technology is under constant development to completely deliver error-free and high quality reads, it has demonstrated a great potential especially in wide-genome analyses. In...

Erratum: A reference bacterial genome dataset generated on the MinIONTM portable single-molecule nanopore sequencer

[This corrects the article DOI: 10.1186/2047-217X-3-22.].

16S rRNA gene sequencing on a benchtop sequencer: accuracy for identification of clinically important bacteria

AIMS Test the choice of 16S rRNA gene amplicon and data analysis method on the accuracy of identification of clinically important bacteria utilizing a benchtop sequencer. METHODS AND RESULTS Nine 16S rRNA amplicons were tested on an Ion Torrent PGM to identify 41 strains of clinical importance. The V1-V2 region identified 40 of 41 isolates to the species level. Three data analysis methods wer...